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Description
Mouse F3 (Coagulation Factor 3) ELISA KitThe Mouse (F3) Coagulation Factor 3 ELISA Kit measures coagulation factor 3 in Mouse samples. The plate has been pre coated with Mouse F3 antibody. F3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse F3 Antibody is added and binds to F3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated F3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
The Mouse (F3) Coagulation Factor 3 ELISA Kit measures coagulation factor 3 in Mouse samples. The plate has been pre-coated with Mouse F3 antibody. F3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse F3 Antibody is added and binds to F3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated F3 antibody. After incubation unbound Streptavidin- HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse F3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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