SKU: 38271379003

Sheep Leptin ELISA Kit

Sale price$343.80 Regular price$382.00
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Ships within 48 hours · Estimated delivery Jul 15 - Jul 20

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Description

Sheep Leptin ELISA KitThe Sheep Leptin ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to sheep LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to sheep LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those

The Sheep Leptin ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to sheep LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to sheep LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain sheep LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of sheep LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 38271379003

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