SKU: 59884016783

Rat MDA (Malonaldehyde) ELISA Kit

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Description

Rat MDA (Malonaldehyde) ELISA KitThe Rat MDA (Malonaldehyde) ELISA Kit measures MDA in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with MDA. MDA in the sample or standard competes with a fixed amount of biotin labeled MDA for sites on a pre coated antibody specific to MDA. Free components are washed off. After adding HRP

The Rat MDA (Malonaldehyde) ELISA Kit measures MDA in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with MDA. MDA in the sample or standard competes with a fixed amount of biotin-labeled MDA for sites on a pre-coated antibody specific to MDA. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of MDA in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 59884016783

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4.5 ★★★★★
Based on 13 reviews
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R
Ree
Phoenix, US
★★★★★ 4
More work than it’s worth
It was great quality and the weight was nice, but it was odd having the open bottom and no spout to dribble into a cup when making an espresso. It was difficult to get the wire tool out of the end of the handle, making it difficult to use. I was not that impressed and it’s quite expensive.
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Reviewed in the United States on March 5, 2025
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Verified Purchase
Mike Steven
New York, US
★★★★★ 5
Quality and Function
Looks like a silly little thing, I am not a coffee enthusiast. But it does exactly what it is supposed to do and with high quality. The device (a stretch to call it that) is made of sturdy materials including the needles. They give you two different thicknesses of needles, one for expressi (0.3) and one set for coffee grinds (0.4). Each comes wth some spares. The stand is well designed and keeps grinds from spreading all over. It is very elegant design. I use this with a dosing funnel on my portafilter. Not the cheapest one could buy but well worth the money.
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Reviewed in the United States on March 16, 2025
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Verified Purchase
Sunny
Waukegan, US
★★★★★ 5
Well made, high quality product.
This product demonstrates excellent quality, featuring a robust construction and intuitive design for ease of use. It includes two distinct wire sizes and offers straightforward assembly. Its superior durability and strong magnetic properties ensure effortless reattachment to its stand.
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Reviewed in the United States on June 30, 2025
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Verified Purchase
Amazon Customer
Charlottesville, US
★★★★★ 5
Perfect WDT tool
Best WDT I’ve found. Love that it includes 0.3mm needles (as I’ve found that 0.4mm needles push the grounds around too much), and the stand is very clever. It knocks off the residual grounds on the needles and is easy to use. As far as WDT tools go, I can’t imagine anything better.
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Reviewed in the United States on April 13, 2025
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Verified Purchase
MrEdmonds
Pawtucket, US
★★★★★ 5
Amazing quality build
This WDT tool is solid and well-made. Definitely worth having!
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Reviewed in the United States on June 26, 2025

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