SKU: 40465192759

Rat PTH ELISA Kit

Sale price$192.85 Regular price$214.28
Save 10%

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 11 - Jul 16

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Rat PTH ELISA KitProduct Specification protein PTH Usage 1. Sample handling and requirements 1. Serum: Whole blood samples collected in serum separation tubes were placed at room temperature 2 Hour or 4 Overnight, then 1000g Centrifugation 20 Minutes, take the supernatant, or place the supernatant in 20 Or 80 Store, but repeated freezing and thawing should be avoided. 2. Plasma: use EDTA Or heparin as an anticoagulant to collect specimens, and collect the specimens

Product Specification

protein PTH
Usage

1. Sample handling and requirements

1. Serum:
Whole blood samples collected in serum separation tubes were placed at room temperature 2 Hour or 4℃ Overnight, then 1000×g Centrifugation 20 Minutes, take the supernatant, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided.

2. Plasma:
use EDTA Or heparin as an anticoagulant to collect specimens, and collect the specimens after collection 30 Within minutes 2-8℃ 1000×g Centrifugation 15 Minutes, take the supernatant to detect, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided.

3. Tissue homogenate:
With pre-cooled PBS (0.01M, pH=7.4) The tissue is flushed to remove residual blood (lysed red blood cells in the homogenate can affect the measurement), and the tissue is weighed and cut into pieces. Combining the shredded tissue with the corresponding volume PBS (Generally according to 1:9 Weight to volume ratio, such as 1g The tissue samples correspond to 9mL Of PBS The specific volume can be appropriately adjusted according to the experimental needs and recorded. Recommended in PBS Add protease inhibitor) into a glass homogenizer and ground thoroughly on ice. For further lysis of tissue cells, the homogenate can be sonicated, or freeze-thawed repeatedly. Finally, the homogenate was mixed in 5000×g Centrifugation 5~10 Minutes,
Take the supernatant for detection.

4. Cell culture supernatant or other biological specimen:
Please 1000×g Centrifugation 20 Minutes, take the supernatant to detect, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided.

Note: Hemolysis of the specimen will affect the final test result, so hemolyzed specimens should not be tested.

Two, Reagent Preparation

The kit should be removed from the refrigerated environment and equilibrated at room temperature before use.

20× Dilution of wash buffer: distilled water according to 1 : 20 Dilution, i.e. 1 Share 20× Wash buffer plus 19 Distilled water.

 

Three, Operation steps

1. Equilibration from room temperature 20min
Take out the required slats from the aluminum foil bag after that, and seal the remaining slats with ziplock bag and put them back 4℃ 。

2. Set up standard wells and sample wells, add different concentrations of standards to each standard well 50μL ;

3. The sample to be tested is added to the sample well 50μL ;
Blank holes are not added.

4. In addition to the blank wells, horseradish peroxidase ( HRP ) Labeled detection antibody
100μL Sealing the reaction hole with a sealing plate membrane, 37℃ Incubation in water bath or incubator 60min 。

5. Discard the liquid, pat dry on absorbent paper, and fill each well with wash liquid ( 350μL ), let stand 1min , throw off the washing liquid, pat dry on absorbent paper, and repeat washing the plate 5 Times (the plate can also be washed with a plate washing machine).

6. Substrate added per well A 、 B  Each 50μL , 37℃ Incubate in the dark 15min 。

7. Add stop solution to each well 50μL , 15min  Inside, in 450nm Wavelength measurement of each well OD  Value.

 

Calculation of experimental results

Based on the tested standard OD The value is the abscissa, and the concentration value of the standard product is the ordinate. Draw the standard curve on coordinate paper or with relevant software, and obtain the linear regression equation. The sample OD Values are substituted into Eq., and the concentration of the sample is calculated.


Standard curve

Species Reactivity Rat
Theory The kit uses a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with rat parathyroid hormone (PTH) capture antibody, specimens, standards, and HRP-labeled detection antibodies were sequentially added, incubated and thoroughly washed. The color is developed with the substrate TMB, which is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. There was a positive correlation between the depth of color and rat parathyroid hormone (PTH) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the sample concentration was calculated.
Source Rat
Synonym Rat Parathyroid Hormone (PTH) Kit (ELISA)
Composition

Name

96T

remark

Microwell microplate

12 Hole ×8 Strip

without

Standard

0.3mL*6 Tube

without

Sample dilution

6mL

without

Detection antibodies -HRP

10mL

without

20× Wash buffer

25mL

Dilute as per instructions

Substrate A

6mL

without

Substrate B

6mL

without

Stop liquid

6mL

without

Sealing film

2 Zhang

without


remark

1. The standard concentrations are as follows: 2000 、 1000 、 500 、 250 、 125 、 62.5 pg/mL

2. After inspection of a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit, which are directly taken during the experiment 50μL Just load the sample. When some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before conducting the experiment.
General Notes

1. Incubate in strict accordance with the specified time and temperature to ensure accurate results. All reagents must reach room temperature 20-25 °C prior to use. Store reagents in refrigeration immediately after use.

2. Incorrect plate washing can lead to inaccurate results. Make sure to drain the liquid from the wells as much as possible before adding the substrate. Do not allow the wells to dry out during incubation.

3. Eliminate the residual liquid and fingerprints at the bottom of the plate, otherwise it will affect the OD value.

4. The substrate color development solution should be colorless or very light in color, and the substrate solution that has turned blue cannot be used.

5. Avoid cross-contamination of reagents and specimens to avoid wrong results.

6. Avoid direct exposure to strong light during storage and incubation.

7. After equilibrating to room temperature, open the sealed bag to prevent water droplets from condensing on the cold slats.

8. Any reaction reagent cannot come into contact with the bleaching solvent or the strong gas emitted by the bleaching solvent. Any bleaching component will destroy the biological activity of the reaction reagents in the kit.

9. Expired products cannot be used.

10. If it is possible to spread diseases, all samples should be managed, and the samples and testing devices should be handled according to the prescribed procedures.

Storage Temp. Unopened kit, stored at 4 °C, shelf life 6 months.
Test Range 62.5 pg/mL-2000 pg/mL; Sensitivity: The lowest detection concentration is less than 10 pg/mL.
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 40465192759

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.1 ★★★★★
Based on 2268 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
K
Verified Purchase
Kristi's Reviews
Draper, US
★★★★★ 5
Surprisingly Effective and Convenient!
Color: Bag Petrol
I wasn’t sure what to expect with the purse clean ball, but it has turned out to be a great little gadget! It’s simple to use—just toss it into your purse, and it quietly works to pick up crumbs, lint, and small debris. The sticky interior does an excellent job of collecting all the random dirt that accumulates, and it’s easy to clean—just rinse it under water, let it dry, and it’s good as new. I also love that it’s compact and doesn’t take up much space in my bag. If you want an easy way to keep your purse tidy without constantly emptying and shaking it out, this is a fun and effective solution. Highly recommend it for anyone who likes to keep their bags neat and clean!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 20, 2024
M
Verified Purchase
Meghan Amsberry
Lake Worth, US
★★★★★ 5
Highly recommend
Color: Black
I love these so much. They make the perfect stocking stuffer but as a chronically messy person, I love throwing them in my purse or backpack. They pick up so much and are really easy to wash off.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2026
S
Verified Purchase
sunshine4378
Grantham, US
★★★★★ 4
Clean Your Purse
Color: Pink
This ball is pretty cool. It acts like a lint roller inside your purse getting all the dirt, crumbs, & lint that accumulates at the bottom. When it's looking nasty, you can rinse it off in the sink & it's good to go back in your purse. It's one of my best Amazon purchases and I will probably buy again if/when the sticky wears off or having a spare on hand.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 29, 2025
J
Verified Purchase
John C
Draper, US
★★★★★ 5
Value for money
Color: Blue
This definitely is something to have. Used it a lot while travelling and for office use as well. It takes out all the gunk from the bag and is easy to clean as well
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 8, 2026
D
Verified Purchase
David
Natrona Heights, US
★★★★★ 5
Used for a year.
Color: Black
Had this in a back pack for a year and wow. The dirt collected is kinda shocking. I will buy more of these for different bags!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 21, 2026

recommand products