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Description
Human MSR1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Macrophage Scavenger Receptor 1 (MSR1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Macrophage Scavenger Receptor 1 (MSR1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Macrophage Scavenger Receptor 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Macrophage scavenger receptor 1, also known as MSR1, is a protein encoded by the MSR1 gene. MSR1 is also designated CD204 (cluster of differentiation 204). This gene encodes a class A macrophage scavenger receptor, which includes three different types (1, 2, and 3) generated by alternative splicing of the gene. These receptors or isoforms are trimeric integral membrane glycoproteins that have been implicated in many macrophage-related physiological and pathological processes, including atherosclerosis, Alzheimer's disease, and host defense. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.8 ★★★★★
Based on 1553 reviews
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Product Reviews
★★★★★ 4
Very nice suit for wedding or other event.
Size: Large, Color: Navy Blue, Size: Large, Color: Navy Blue
The jacket was a perfect fit, the pants were a little long. Material was nice, and well put together. Overall I was very pleased with this suit for my wedding.
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Reviewed in the United States on September 24, 2025
★★★★★ 5
Amazing suit for the price
Size: Large, Color: Black
So I didn't really need a suit for anything. But I've been wanting one just in case. As such, I wasn't willing to spend a lot. Yet I still wanted to look good when I wore it.
This suit is amazing. I honestly would fit between a medium and a large, and with my current trend down in weight, I may buy an additional in medium(I mean, for under $100, why not?).
While my shoulders have always been a little wide for what fits my waist, the large seems to accommodate even more than I need. I worry if the medium will be too tight, but that's for another day.
The pants are a little long, to be expected considering I generally wear 30' pants. Easy to hem.
The vest seemed a little loose, until I found the adjustment in the back. Now it is perfect.
I may still need to go to a tailor for a more custom fit, but for the price it can't be beat. And I of course haven't tied a tie since I was 16, so time to relearn that.
As far as quality goes, the fabric and stitching all appear to be excellent. I went with the black, as I don't particularly look great in gray, and navy out here seems more suited for church than anything else. The quality of black is excellent.
The quality of the pants is good, but I will still be favoring my dress pants that already fit well.
I especially love the angle of the beast cut(whatever it is called). Whether I choose the vest or not, the black contrast with all of my dress shirts is fantastic.
I will include photos sometime when I'm not too self-conscious about taking selfies.
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Reviewed in the United States on June 22, 2024
★★★★★ 5
Really amazing suit that looks like a million bucks!
Size: Large, Color: Black
This is the second suit we bought by Lupurty. The first was dark navy and fit so well and looked so good that we got one in black for our son’s wedding!
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Reviewed in the United States on March 30, 2026
★★★★★ 1
Disgusted!
Size: Small, Color: Black
I’m disgusted! I ordered this for my boyfriend to wear to prom. I ordered him a small and the jacket and the vest were not smalls, they look like they were a large. All three pieces had dog or cat hair on them and the jacket had dirt stains on the inside. They were all used! And some of the stitching was coming out! I’m so glad I received it the same day I bought it, because if received it closer to prom I would have been so screwed!!! Do not buy this or buy from this brand! Terrible product!!
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Reviewed in the United States on April 10, 2026
★★★★★ 5
Dress to Impress: Sharp, Tailored Fit, Quality Material, and Sizing Tips
Size: X-Small, Color: Navy Blue, Size: X-Small, Color: Navy Blue
This review is for the LUPURTY Men's Suits Slim Fit, 3 Piece Suits for Men, 2 Buttons Solid Jacket Vest Pants, Tuxedo Set (XS navy blue and black sets).
I bought the XS knowing my son's measurements were actually below the size guide for that size, but I'm glad I did because it still worked out. The suit was a bit big—especially in the pants, which were quite long, and slightly roomy in the shoulders and arms—but it still looked sharp and well put-together. The overall fit has a clean, tailored appearance despite the extra room. The material feels high-quality and the color is true to what’s shown online. I do wish the black suit came with a black or gray tie instead of blue, which didn’t quite match.
The suit in the photos is the navy blue set that my son wore to his 8th grade graduation, which matched their school colors perfectly.
I also included my son’s measurements to help others with sizing:
Weight 114 lbs
Height 5'3"
Chest 30.5"
Waist 29"
Hip 35"
Sleeve length 21.5"
Shoulder 17"
Inseam 29"
Back length 18.5"
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Reviewed in the United States on July 9, 2025