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Description
Human FUT2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against galactoside 2-alpha-L-fucosyltransferase 2 (FUT2). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of galactoside 2-alpha-L-fucosyltransferase 2 (FUT2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Galactoside 2-alpha-L-fucosyltransferase 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Fucosyltransferase 2, also known as FUT2, is an enzyme encoded by the FUT2 gene. FUT2 is a phospholipid peroxidase that protects cells from membrane lipid peroxidation. The antioxidant enzyme fucosyltransferase 2 (FUT2) belongs to the glutathione peroxidase family, which consists of eight known mammalian isoenzymes (GPX1-8). FUT2 catalyzes the reduction of hydrogen peroxide, organic hydroperoxides, and lipid peroxides at the expense of reduced glutathione and plays a role in protecting cells from oxidative stress. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
Based on 2246 reviews
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Product Reviews
★★★★★ 5
The perfect throw-on athletic dress (with built-in shorts!)
Size: Small, Color: Navy Blue
I’ve been loving this athletic dress—it’s one of those pieces that’s both functional and cute, which is hard to find. The fabric is lightweight, breathable, and slightly stretchy, so it’s comfortable whether you’re running errands, going for a walk, or just chasing kids around all day.
The built-in shorts are a huge plus. They stay in place well and make it feel more secure than a regular dress, especially on busy days. I also appreciate the pockets—they’re actually usable and don’t feel bulky.
The fit is relaxed and flowy, which I personally like because it’s easy and flattering without clinging. The straps are adjustable, so you can customize the fit a bit depending on your height or preference.
I’d say it runs true to size for a looser fit. If you want it more fitted, you could size down, but I like the casual, sporty look as is.
Overall, this is a great everyday summer staple—super easy to style with sneakers or sandals, and perfect for travel or on-the-go days. I’ll definitely be getting more colors!
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Reviewed in the United States on April 24, 2026
★★★★★ 5
Perfect for summer days.
Size: Medium, Color: Deep Gray, Size: Medium, Color: Deep Gray
I absolutely love this little athletic summer dress. I typically wear a size ten and ordered this in a medium and it's a perfect fit. I have the straps short so way it doesn't look baggy. It is super soft and comfortable. It is breathable and a perfect cut and length for me so so I plan on wearing it all during hot summer days. This is very versatile and easy to style by layering different things underneath it. I have a gray and leopard sports bra that is a really close shade of gray so it almost looks like it's one piece. I plan to order the teal as I have a swimsuit top that will match that color spot on. It seems like it is good quality and should hold up well to normal usage. I like that it has really big pockets , but I likely will not use them often lol. I just like the fact that if I need to have pockets they are there.
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Reviewed in the United States on April 15, 2026
★★★★★ 4
Size down! But overall comfortable and cute and good quality
Size: Small, Color: Light Yellow
Overall it’s cute and comfortable, but my advice is to SIZE DOWN!!! I wish I would have, but I’ve already washed it so it’s too late. The straps are adjustable, but even so, I’ve had to tighten them as far as they’ll go. For reference, I am 5’1” and 125#, rectangle shaped/boxy body with a very small chest and I got a Small. The top gapes open on me and the dress is longer than I prefer for this style. Material/fabric is nice and good quality and it’s a perfect one-piece easy outfit for warm days. I like the yellow color too. It is tricky to find the right bra to wear with it, though. Got lots of compliments on it the first time I wore it.
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Reviewed in the United States on April 22, 2026
★★★★★ 5
Cute, Comfy & Flowy—Great for Plus Size!
Size: XX-Large, Color: Black
I really like this dress!
It’s super comfortable and lightweight, and the material feels soft without being too thin. It has a nice flow to it, so it doesn’t cling to your body, which I really appreciate.
For reference, I’m 5’4” with a 46” bust, and the dress fits well overall. The length is great, and I love that it has a relaxed fit—it’s easy to throw on and still look put together.
The pockets are also a nice bonus and actually usable.
The only thing to note is the top area can be a little more open on the sides, so I personally prefer wearing a sports bra or something cute underneath. It actually works really well styled that way and gives that “layered” look.
Overall, it’s a really easy, comfortable dress that you can dress up or down. I’ll definitely be wearing this a lot!
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Reviewed in the United States on May 20, 2026
★★★★★ 5
Great dress for running around town
Size: Large, Color: Coffee
I love this dress. I ordered this dress fist in a medium, then a large. I probably would have been fine in the large, but I just wanted a little more room and I wanted it to come to my knees so I ordered the XL and I love it. The material is a cotton knit type material similar to a t-shirt with a little stretch to it. I am 5'2", #170 and the XL gave me the fit I was looking for, being loose and comfortable. There is a second layer under the dress that has shorts and a tank top built in. Unfortunately, you must take the entire thing off to use the restroom but I am considering adding snaps to the crotch so that it is easier and I won't have to completely undress to use the restroom. The olive/army green is beautiful and I am planning to order the plum color dress as another addition to my summer wardrobe.
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Reviewed in the United States on May 15, 2026