Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 11 - Jul 16
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human TGFBR2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Transforming Growth Factor Beta Receptor II (TGFBR2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Transforming Growth Factor Beta Receptor II (TGFBR2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transforming Growth Factor Beta Receptor II ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Transforming growth factor beta receptor II (70/80 kDa) is a TGF-beta receptor. It is a tumor suppressor gene. It encodes a member of the serine/threonine protein kinase family and the TGF-beta receptor subfamily. The encoded protein is a transmembrane protein with a protein kinase domain that forms a heterodimeric complex with another receptor protein and binds to TGF-beta. This receptor/ligand complex phosphorylates the protein, which then enters the cell nucleus and regulates the transcription of a subset of genes involved in cell proliferation. Mutations in this gene have been associated with Marfan syndrome, Loeys-Deitz aortic aneurysm syndrome, Osler-Weber-Rendu syndrome, and the development of various types of tumors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 784 reviews
Sort
Product Reviews
★★★★★ 5
Health, Eco, AND Econo friendly.
Style: HCC2128-X
40K+ miles o my 2020 Ranger. Thought I’d replace the CAF (cabin air filter.) Holy c__p. The original factory filter was clogged with dust, mesquite seeds, Palo verde droppings -YUCK. This reusable filter improved airflow immensely, plus the cabin feels fresher. Quite a bargain compared to the major brand reusable CAF (K_N) too, or pulling clogged filters, throwing them in the trash and buying a new disposable filter to install. I’m looking forward to pulling this in a month and seeing what it kept out of my breath, washing it, and reinstalling it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 23, 2025
★★★★★ 5
Great filter
Style: HCC2146-X
My husband said this filter was high-quality, he was quite impressed. We’re not crazy about the washable kind normally, so we’ll have to see how it holds up.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 29, 2026
★★★★★ 5
Filter Fits Properly and is an Easy Install
Style: Particulate Media
Though not made for or by Honda, this filter is inexpensive and looks like it's capable of filtering the air coming into the passenger compartment of our 2012 Honda Fit. The primary difference between this unit and the original filter supplied by Honda is that the original unit has some "ribs" that help to keep the pleats separated attached to the top of the pleats. The item I received lacks that, but it appears to me that the pleats are strong enough on their own for the intended purpose. The filter fits into the frame as it should. Replacing this filter is incredibly easy and requires no tools: you empty the glove compartment, compress it's sides inward so that the rubber stops on either side clear the dash, allowing the glove compartment to drop out an extra 4-5 inches. Then, you can see and reach the cover for the cabin air filter, which is secured by latches on each side. Remove the cover and slide out the filter and frame. Replace the air filter element, which just sits in the frame, replace the frame and cover, and compress the glove compartment sides again to put it back in place. Put your stuff back in the glove compartment and you're done. In a few minutes you've saved significant dollars over the cost of having this done in the shop. Congratulations! The engine air filter is equally easy to replace in the Fit, though for that job I prefer to use a Honda OEM part.
Edit: I thought I should add a reminder to install the filter in such a way that the air flow indicator is the same as the one that you remove, i.e. do not put it in upside down. If I remember correctly, air flows from above to below in the Honda Fit. Also, currently, the price for this item is not competitive.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 21, 2014
★★★★★ 5
Easiest to install so far
Style: HEPA
So the 2018 Honda Odyssey apparently checks the air flow and after a certain point it determines the cabin air flow is less than desirable. At least this this is what I’ve gathered after talking to my dealer. Buying a thick filter may not be better.
This was the easiest filter to install. I didn’t have to gingerly coax it In without collapsing the filter. It fit easily and I didn’t have to cut it. Pro Tip: Write the date of install in case your dealership says “well cabin air flow is blah blah blah.” Just tell them to check the date. You should get 6-12months on a filter.
My dealership wanted to charge a ridiculous amount for what is literally a 2 minute job. Decent filters will cost around $20. Thicker isn’t better. It all depends on how much crap you have in your glove box. And how much crap is in your air. It might take longer to get all the receipts, first aid kits, tick prevention kits, headlights for the Scouts that forgets theirs, oh that there’s that Leatherman and Mylar blanket is…
The job entails emptying the glove box, who’s keeps gloves in these? Press the tabs to drop the box and then Voila there is the filter. It’s really that easy. Ok pull the cover off and pull the old filter. Unless you live in a really dusty environment or have squirrels that are destined to ruin your life there shouldn’t be any real mess . Pull it out and put this in. There’s air flow arrows but even if you did it backwards the van isn’t going to explode in a ball of flame, fueled by the forgotten Cheerios under the seat.
Relax, you did it. The world still turns and you’re good to go. Now it’s time to think about the engine air filter. You can do it. It’s easy but the clips can be a pain so it’s better to grab an adult beverage before, just in case.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 11, 2021
★★★★★ 5
Great Product
Style: HEPA
This product is a fantastic find! It's reasonably priced, making it accessible for most budgets without compromising on quality. The product is exactly as described, and its usefulness cannot be overstated. I've found it to be incredibly practical and efficient in meeting my needs. The attention to detail is impressive, and the performance is on par with more expensive alternatives. If you're looking for something reliable, well-made, and at a fair price point, this is the perfect choice. Highly recommended!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 28, 2026