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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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4.7 ★★★★★
Based on 850 reviews
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Product Reviews
★★★★★ 5
Great basic and timeless design
Size: 12 Little Kid, Color: Brown
These sandals are a great find! They’re super easy to get on and off thanks to the Velcro straps, which is a huge win for little ones learning to be more independent. The fit is adjustable and secure, so they stay in place well during all kinds of play.
The cork-style footbed is comfortable and gives good support, and the overall design feels sturdy enough to handle everyday wear. They’re lightweight but still durable, and have held up really well so far.
They also have a classic, versatile look that goes with just about everything, perfect for summer outfits. Overall, a reliable, comfortable sandal that’s great for active kids. Would definitely buy again!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 24, 2026
★★★★★ 5
Great option for boys or girls
Size: 13 Little Kid, Color: Black, Size: 13 Little Kid, Color: Black
This is really more of a unisex option for a sandal. I have sandals that look like this, and both of my children have worn this style. I ordered these for my daughter, with the plan to pass them down to my son if they're not too worn when she outgrows them. The style is very minimalistic, so it goes with lots of outfits. All the straps are easily adjustable, so you can get a good fit. I would say the length is true to size, but the width is maybe a bit wide. Nevertheless, the adjustable straps really help them fit well. They feel really sturdy and I appreciate that the sole has cushion to it. My daughter has worn these every day since I got them out of the closet and they're holding up well.
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Reviewed in the United States on February 19, 2026
★★★★★ 1
Velcro strap non durable.
Size: 10.5 Little Kid, Color: Oltremare
The Velcro strap stop working after using the shoes for only less than 2 month.
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Reviewed in the United States on July 19, 2025
★★★★★ 5
Daughter approved
Size: 1 Little Kid, Color: Turquoise/Multi
My daughter picked out these shoes and she absolutely loves them.
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Reviewed in the United States on May 9, 2026
★★★★★ 5
Not stiff or tight…
Size: 13.5 Little Kid, Color: Turquoise/Multi
My niece loved them. So many of the slips in shoes are too stiff and tight, this one was easy on, not stiff or tight, just perfect!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 18, 2026
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